C57BL/6-Tcf21tm1(icre/ERT2)Bcgen/Bcgen • 110141
| Product name | B-Tcf21-iCreERT2 mice |
|---|---|
| Catalog number | 110141 |
| Strain name | C57BL/6-Tcf21tm1(icre/ERT2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 21412 |
| Official symbol | Tcf21 (transcription factor 21), Po, ep |
| Chromosome | 10 |
| Species | Mouse |
| Application | Function researchof genes. This Tcf21iCreERT2 model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of Transcription factor 21 expressed during mouse embryogenesis specifically in mesodermally-derived cells that surround the epithelium of the developing gastrointestinal, genitourinary, and respiratory systems, and may be useful for studying mouse embryonic development. |
Targeting strategyA iCreERT2-WPRE-pA sequence cassette was inserted downstream 5’UTR of the Tcf21 gene. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
Blocking SEV secretion from ECs delays AML progression. (A) The experimental design. (B–F) Survival curves of AML recipients with different genotypes. (B) Cdh5-Cre;Vps33bfl/fl, (C) Lepr-Cre;Vps33bfl/fl, (D) Osx-CreER;Vps33bfl/fl, (E) Pf4-Cre;Vps33bfl/fl, and (F) Tcf21-CreER;Vps33bfl/fl are shown (n = 5–8; **P < 0.01, ***P < 0.001, log-rank test). Experiments were conducted 3 times for validation. They used 7 cell type–specific mouse Cre lines to conditionally knock out Vps33b in Cdh5+ or Tie2+ endothelial cells (ECs), Lepr+ BM perivascular cells, Osx+ osteoprogenitor cells, Pf4+ megakaryocytes, and Tcf21+ spleen stromal cells. (source: Huang D et al.,2021)
