Protein expression analysis
Strain specific ADA expression analysis in homozygous B-hADA mice by western blot. Tissues were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hADA mice (H/H), and analyzed by western blot with anti-ADA antibody. ADA was detectable in homozygous B-hADA mice.
mRNA expression analysis
Strain specific analysis of ADA mRNA expression in wild-type C57BL/6 mice and B-hADA mice by RT-PCR. Spleen RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADA mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADA primers. Mouse Ada mRNA was detectable in wild-type C57BL/6 mice. Human ADA mRNA was detectable only in homozygous B-hADA mice but not in wild-type mice.
