Western blot analysis of ADAM9 protein expression in homozygous B-hADAM9 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hADAM9 mice (H/H), and then analyzed by western blot with anti-ADAM9 antibody. 40 μg total proteins were loaded for western blotting analysis. ADAM9 protein was detected in the heart, liver, spleen, lung, kidney, brain and thymus of wild-type C57BL/6 mice and homozygous B-hADAM9 mice, as the antibody is cross-recognize both human and mouse ADAM9.