mRNA expression analysis
Strain specific analysis of APOA1 mRNA expression in wild-type C57BL/6 mice and B-hAPOA1 mice by RT-PCR. Liver RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hAPOA1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APOA1 primers. Mouse Apoa1 mRNA was detectable only in wild-type C57BL/6 mice. Human APOA1 mRNA was detectable only in homozygous B-hAPOA1 mice but not in wild-type mice.
Protein expression analysis in plasma
Strain specific APOA1 expression analysis in homozygous B-hAPOA1 mice by ELISA. Plasma collected from wild-type C57BL/6J mice (+/+) and homozygous B-hAPOA1 mice (H/H) were analyzed by ELISA with species-specific APOA1 ELISA kit. Human APOA1 was exclusively detectable in homozygous B-hAPOA1 mice but not in wild-type mice.
