Gene targeting strategy for B-hCD14 mice.
The exons 1-2 of mouse Cd14 gene that encode the full-length protein were replaced by human CD14 exons 1-2 in B-hCD14 mice.

Strain specific CD14 expression analysis in wild-type C57BL/6 mice and homozygous B-hCD14 mice by flow cytometry. PEMs (peritoneal exudate macrophages) were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD14 mice (H/H). Mouse CD14 was detectable only in wild-type mice. Human CD14 was detectable only in homozygous B-hCD14 mice.

Strain specific analysis of CD14 gene expression in wild-type C57BL/6 mice and B-hCD14 mice by RT-PCR. Mouse Cd14 mRNA was detectable in PEMs (peritoneal exudate macrophages) of wild-type C57BL/6 mice (+/+) but not in homozygous B-hCD14 mice (H/H). Human CD14 mRNA was detectable only in homozygous B-hCD14 mice but not in wild-type C57BL/6 mice.