C57BL/6-Cd22tm1(CD22)Bcgen/Bcgen • 110775
| Product name | B-hCD22 mice |
|---|---|
| Catalog number | 110775 |
| Strain name | C57BL/6-Cd22tm1(CD22)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 12483 |
| Aliases | cluster of differentiation-22, SIGLEC2 |
Targeting strategyGene targeting strategy for B-hCD22 mice. The exons 4~11 of mouse Cd22 gene that encode the extracellular domain were replaced by human CD22 exons 2~9 in B-hCD22 mice.
protein expression analysis
Strain specific CD22 expression analysis in heterozygous B-hCD22 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD22 (H/+) mice, and analyzed by flow cytometry with species-specific CD22 antibody. Mouse CD22 was detectable in WT mice and heterozygous B-hCD22 mice. Human CD22 was exclusively detectable in heterozygous B-hCD22 mice but not in WT mice.
Strain specific CD22 expression analysis in heterozygous B-hCD22 mice by flow cytometry. Blood were collected from WT and heterozygous B-hCD22 (H/+) mice, and analyzed by flow cytometry with species-specific CD22 antibody. Mouse CD22 was detectable in WT mice and heterozygous B-hCD22 mice. Human CD22 was exclusively detectable in heterozygous B-hCD22 mice but not in WT mice.
mRNA expression analysis
protein expression analysis
Strain specific CD22 expression analysis in homozygouse B-hCD22 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD22 (H/H) mice, and analyzed by flow cytometry with species-specific CD22 antibody. Mouse CD22 was detectable in WT mice. Human CD22 was exclusively detectable in homozygous B-hCD22 mice but not in WT mice.
