C57BL/6-Cd3etm2(CD3E)Bcgen Cd276tm1(CD276)Bcgen/Bcgen • 112617
| Product name | B-hCD3E/hB7-H3 mice |
|---|---|
| Catalog number | 112617 |
| Strain name | C57BL/6-Cd3etm2(CD3E)Bcgen Cd276tm1(CD276)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | CD3E, CD3epsilon, IMD18, T3E, TCRE, CD3 epsilon subunit of T-cell receptor complex; CD276, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, CD276 molecule |
Targeting strategyGene targeting strategy for B-hCD3E/hB7-H3 mice.
The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hCD3E/hB7-H3 mice.
The exon 3-4 of mouse Cd276 gene that encode the extracellular domain were replaced by human CD276 exon 3-6 in B-hCD3E/hB7-H3 mice.
Protein expression analysis
Strain specific CD3E expression analysis in homozygous B-hCD3E/hB7-H3 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hB7-H3 mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H) but not in WT mice (+/+).
mRNA and protein expression analysis
Strain specific analysis of B7-H3 expression in wild type (WT) mice and B-hCD3E/hB7-H3 mice by RT-PCR and WB. Lung were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hB7-H3 mice (H/H; H/H), and analyzed by RT-PCR and western blot with anti-human B7-H3 antibody. Mouse B7-h3 mRNA was detectable only in lung of WT mice (+/+). Human B7-H3 mRNA was detectable only in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing. Human B7-H3 was exclusively detectable in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H).
