C57BL/6-Fastm1(FAS)Bcgen/Bcgen • 111970
| Product name | B-hCD95 mice |
|---|---|
| Catalog number | 111970 |
| Strain name | C57BL/6-Fastm1(FAS)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | FAS, APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, Fas cell surface death receptor |
Targeting strategyGene targeting strategy for B-hCD95 mice.
The exons 2-7 of mouse Fas gene that encode the extracellular domain and transmembrane domain were replaced by human FAS exons 2-7 in B-hCD95 mice.
Protein expression analysis in T cells
Strain specific CD95 expression analysis in homozygous B-hCD95 mice by flow cytometry. Splenocytes were collected from wild-type (WT) mice (+/+) and homozygous B-hCD95 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD95 antibody. Mouse CD95 was detectable in WT mice (+/+). Human CD95 was exclusively detectable in homozygous B-hCD95 mice (H/H) but not in WT mice (+/+).
Protein expression analysis in granulocytes
Strain specific CD95 expression analysis in homozygous B-hCD95 mice by flow cytometry. Splenocytes were collected from wild-type (WT) mice (+/+) and homozygous B-hCD95 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD95 antibody. Mouse CD95 was detectable in WT mice (+/+). Human CD95 was exclusively detectable in homozygous B-hCD95 mice (H/H) but not in WT mice (+/+).
Protein expression analysis in monocytes
Strain specific CD95 expression analysis in homozygous B-hCD95 mice by flow cytometry. Splenocytes were collected from wild-type (WT) mice (+/+) and homozygous B-hCD95 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD95 antibody. Mouse CD95 was detectable in WT mice (+/+). Human CD95 was exclusively detectable in homozygous B-hCD95 mice (H/H) but not in WT mice (+/+).
Protein expression analysis in macrophages
Strain specific CD95 expression analysis in homozygous B-hCD95 mice by flow cytometry.
Splenocytes were collected from wild-type (WT) mice (+/+) and homozygous B-hCD95 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD95 antibody. Mouse CD95 was detectable in WT mice (+/+). Human CD95 was exclusively detectable in homozygous B-hCD95 mice (H/H) but not in WT mice (+/+).
