B-hFGL1 mice

C57BL/6-Fgl1tm1(FGL1)Bcgen/Bcgen • 110116

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B-hFGL1/hLAG3 plus mice

B-hFGL1 mice

Product nameB-hFGL1 mice
Catalog number110116
Strain nameC57BL/6-Fgl1tm1(FGL1)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID234199
AliasesFibrinogen-like protein 1 (FGL-1)
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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hFGL1 mice. The exons 2~8 of mouse Fgl1 gene that encode the full-length protein were replaced by human FGL1 exons 2~8 in B-hFGL1 mice.

      mRNA expression analysis
      Strain specific analysis of FGL1 gene expression in WT and heterozygous B-hFGL1 mice by RT-PCR. Mouse Fgl1 mRNA was detectable in liver cells of wild-type (+/+) mice and heterozygous B-hFGL1 mice. Human FGL1 mRNA was detectable only in H/+, but not in +/+ mice.
      Protein expression analysis
      Strain specific analysis of FGL1 expression in WT and B-hFGL1 mice by western blot. Liver were collected from WT mice and heterozygous B-hFGL1 (H/+) mice. Mouse FGL1 was detectable in WT mice and heterozygous B-hFGL1. Human FGL1 was exclusively detectable in heterozygous B-hFGL1 but not WT mice.