Gene targeting strategy for B-hFZD5 mice. The exon 2 of mouse Fzd5 gene that encodes the full-length protein was replaced by human FZD5 exon 2 in B-hFZD5 mice.

Strain specific FZD5 expression analysis in homozygous B-hFZD5 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFZD5 mice (H/H), and then analyzed by western blot with anti-FZD5 antibody. 40 μg total proteins were loaded for western blotting analysis. FZD5 was detected in brain, as the antibody is cross-recognize both human and mouse FZD5.