B-hGPR55 mice

C57BL/6N-Gpr55tm1(GPR55)Bcgen/Bcgen • 112462

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B-hGPR55 mice

Product nameB-hGPR55 mice
Catalog number112462
Strain nameC57BL/6N-Gpr55tm1(GPR55)Bcgen/Bcgen
Strain backgroundC57BL/6N
AliasesLPIR1
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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hGPR55 mice. The exon 2 of mouse Gpr55 gene that encodes the full-length protein was replaced by human GPR55 exon 2 in B-hGPR55 mice.

      mRNA expression analysis
      Strain specific analysis of GPR55 mRNA expression in wild-type C57BL/6 mice and B-hGPR55 mice by RT-PCR. Brain RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hGPR55 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR55 primers. Mouse Gpr55 mRNA was detectable only in wild-type C57BL/6 mice. Human GPR55 mRNA was detectable only in homozygous B-hGPR55 mice but not in wild-type mice.
      Protein expression analysis

      Western blot analysis of GPR55 protein expression in homozygous B-hGPR55 mice. Various tissue lysates were collected from C57BL/6N wildtype (+/+) mice and homozygous B-hGPR55 (H/H) mice, and then analyzed by western blot with anti-GPR55 antibody. 40 μg total proteins were loaded for western blotting analysis. GPR55 was detected in brain, pancreas, liver and stomach in homozygous B-hGPR55 mice. （The arrow above indicates the glycosylated GPR55 protein, the arrows below indicates the GPR55 protein）