B-hHCAR1 mice

C57BL/6N-Hcar1tm1(HCAR1)Bcgen/Bcgen • 111919

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B-hHCAR1 mice

Product nameB-hHCAR1 mice
Catalog number111919
Strain nameC57BL/6N-Hcar1tm1(HCAR1)Bcgen/Bcgen
Strain backgroundC57BL/6N
AliasesHCAR1 (FKSG80, GPR104, GPR81, HCA1, LACR1, TA-GPCR, TAGPCR)
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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hHCAR1 mice. The exon 1 of mouse Hcar1 gene that encodes the full-length protein was replaced by human HCAR1 exon 1 in B-hHCAR1 mice.

      mRNA expression analysis
      Strain specific analysis of HCAR1 gene expression in wild-type C57BL/6 mice and B-hHCAR1 mice by RT-PCR. Mouse Hcar1 mRNA was detectable only in BAT (brown adipose tissue) of wild-type C57BL/6 mice (+/+). Human HCAR1 mRNA was detectable only in homozygous B-hHCAR1 mice (H/H), but not in wild-type mice.
      Protein expression analysis
      Strain specific HCAR1 expression analysis in homozygous B-hHCAR1 mice by flow cytometry. BAT (brown adipose tissue) was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hHCAR1 mice (H/H), and analyzed by western blot with anti-HCAR1 antibody. HCAR1 was both detectable in wild-type mice and homozygous B-hHCAR1 mice (H/H) due to the cross-reactivity of antibodies.