C57BL/6-Icosltm1(ICOSLG)Bcgen/Bcgen • 110037
| Product name | B-hICOSL mice |
|---|---|
| Catalog number | 110037 |
| Strain name | C57BL/6-Icosltm1(ICOSLG)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | ICOSL, AU044799, B7-H2, B7RP, B7RP-1, B7h, BG071784, GI50, GL5, GL50, GL50-, GL50-B, IC, ICOS-L, Icoslg, L, LICOS, Ly115l, mKIAA0653, icos ligand |
Targeting strategyGene targeting strategy for B-hICOSL mice. The exons 3-5 of mouse Icosl gene that encode the extracellular domain were replaced by human ICOSL exons 3-5 in B-hICOSL mice.
Protein expression analysis
Strain specific ICOSL expression analysis in heterozygous B-hICOSL mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and heterozygous B-hICOSL mice (H/+), and analyzed by flow cytometry with anti-mouse ICOSL antibody and prezalumab analog (in house). Mouse ICOSL was detectable in WT mice (+/+) and heterozygous B-hICOSL mice (H/+). Human ICOSL was exclusively detectable in heterozygous B-hICOSL mice (H/+) but not in WT mice (+/+).
Strain specific ICOSL expression analysis in heterozygous B-hICOSL mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and heterozygous B-hICOSL mice (H/+), and analyzed by flow cytometry with anti-human ICOSL antibody A and antibody B provided by the client. Mouse ICOSL was detectable in WT mice (+/+) and heterozygous B-hICOSL mice (H/+). Human ICOSL was exclusively detectable in heterozygous B-hICOSL mice (H/+) but not in WT mice (+/+).
