B-hIL4/hIL4RA/hTSLP/hTSLPR plus mice were derived from mating B-hIL4/hIL4RA mice(120551) and B-hTSLP/hTSLPR mice plus(112744). For validation data of this mouse model, you can refer to the validation data from the related gene humanized mouse models.

Gene targeting strategy for B-hIL4/hIL4RA/hTSLP/hTSLPR plus mice. The exons 1-5 of mouse Tslp gene that encode the full-length protein were replaced by human TSLP exons 1-4 in B-hTSLP/hTSLPR mice plus. The signal peptide, extracellular and transmembrane region of human TSLPR gene and the cytoplasmic region of mouse Tslpr gene were constructed into a chimeric CDS vector and inserted into the mouse exon 2.The targeted mice will express the chimeric TSLPR protein, while mouse TSLPR will no longer express in B-hTSLP/hTSLPR mice plus. The exons 1-4 of mouse Il4 gene that encode the full length coding sequence were replaced by human IL4 exons 1-4 in B-hIL4/hIL4RA mice. The exons 4-7 of mouse Il4ra gene that encode the extracellular region coding sequences were replaced by human IL4RA exons 4-7 in B-hIL4/hIL4RA mice. B-hIL4/hIL4RA/hTSLP/hTSLPR plus mice was developed by cross-mating the B-hTSLP/hTSLPR mice plus and the B-hIL4/hIL4RA mice together.