Strain specific analysis of MCAM gene expression in wild type mice and B-hMCAM mice by RT-PCR. Kidney RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hMCAM mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human MCAM primers. Mouse Mcam mRNA was detectable only in kidney of wild type mice (+/+). Human MCAM mRNA was detectable only in homozygous B-hMCAM mice (H/H) but not in wild type mice (+/+).

Strain specific MCAM expression analysis in homozygous B-hMCAM mice by flow cytometry. Kidney, small intestine and testis were collected from wild type mice (+/+) and homozygous B-hMCAM mice (H/H), and analyzed by flow cytometry with species-specific anti-MCAM antibody. Mouse MCAM was detectable in wild type mice (+/+). Human MCAM was detectable in homozygous B-hMCAM mice (H/H).