Strain specific analysis of MCEMP1 mRNA expression in wild-type C57BL/6 mice and B-hMCEMP1 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hMCEMP1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human MCEMP1 primers. Mouse Mcemp1 mRNA was detectable in wild-type C57BL/6. Human MCEMP1 mRNA was detectable only in homozygous B-hMCEMP1 mice but not in wild-type mice.

Western blot analysis of MCEMP1 protein expression in homozygous B-hMCEMP1 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hMCEMP1 mice (H/H), and then analyzed by western blot with anti-MCEMP1 antibody (abcam, ab121447). 40 μg total proteins were loaded for western blotting analysis. MCEMP1 was detected in lung, heart, spleen and kidney in wild-type mice and homozygous B-hMCEMP1 mice, as the antibody is cross-recognized between human and mouse MCEMP1.