C57BL/6-Pdcd1tm1(PDCD1)BcgenCd274tm1(CD274)BcgenCd3etm2(CD3E)Bcgen/Bcgen • 111923
| Product name | B-hPD-1/hPD-L1/hCD3E mice |
|---|---|
| Catalog number | 111923 |
| Strain name | C57BL/6-Pdcd1tm1(PDCD1)BcgenCd274tm1(CD274)BcgenCd3etm2(CD3E)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | PDCD1, programmed cell death 1, PD-1; CD274, CD274 molecule, PD-L1; CD3E, IMD18, T3E, TCRE,CD3e molecule |
Targeting strategyGene targeting strategy for B-hPD-1/hPD-L1/hCD3E mice. The exon 2 of mouse Pdcd1 gene that encodes the IgV domain was replaced by human PDCD1 exon 2 in B-hPD-1/hPD-L1/hCD3E mice. The exon 3 of mouse Cd274 gene that encodes the IgV domain was replaced by human CD274 exon 3 in B-hPD-1/hPD-L1/hCD3E mice. The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hPD-1/hPD-L1/hCD3E mice.
Protein expression analysis in T cells
Strain specific PD-1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild-type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-1 antibodies. Mouse PD-1 was detectable in WT mice (+/+). Human PD-1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific CD3E expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild-type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibodies. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific PD-L1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild-type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-L1 antibodies. Mouse PD-L1 was detectable in WT mice (+/+). Human PD-L1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).
Protein expression analysis in dendritic cells
Strain specific PD-L1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild-type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-L1 antibodies. Mouse PD-L1 was detectable in WT mice (+/+). Human PD-L1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).
