B-hSERPINE2 mice

C57BL/6-Serpine2tm1(SERPINE2)Bcgen/Bcgen • 111271

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B-hSERPINE2 mice

Product nameB-hSERPINE2 mice
Catalog number111271
Strain nameC57BL/6-Serpine2tm1(SERPINE2)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID20720
AliasesGDN, GDNPF, PI-7, PI7, PN-1, PN1, PNI
ApplicationThis product is used for pharmacodynamics and safety evaluation of antithrombotic drugs and antitumor drugs.
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  • Description
  • Targeting strategy
  • Phenotypic analysis

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      Description
      • The SERPINE2 gene encodes the PN-1 protein, which belongs to the serpin family and is a secreted protein.
      • The exons 2~9 of mouse Serpine2 gene that encode the full-length protein were replaced by human SERPINE2 exons 2~9 in B-hSERPINE2 mice.
      • SERPINE2 protein was detected in brain, lung, spleen and ovary of homozygous B-hSERINE2 mice.
      Targeting strategy

      Gene targeting strategy for B-hSERPINE2 mice. The exons 2~9 of mouse Serpine2 gene that encode the full-length protein were replaced by human SERPINE2 exons 2~9 in B-hSERPINE2 mice.

      mRNA expression analysis

      Strain specific analysis of SERPINE2 gene expression in wild-type mice and B-hSERPINE2 mice by RT-PCR. Mouse SERPINE2 mRNA was detectable in lung of wild-type mice (+/+). Human SERPINE2 mRNA was detectable only in lung of homozygous B-hSERPINE2 but not in wild-type mice.

      Western blot analysis of SERPINE2 protein expression in homozygous B-hSERPINE2 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSERPINE2 mice (H/H), and then analyzed by western blot with species-specific anti-SERPINE2 antibody. 40 μg total proteins were loaded for western blotting analysis. SERPINE2 was detected in brain, spleen, lung, and ovary.