C57BL/6-Sirpatm1(SIRPA)Bcgen Cd47tm1(CD47)Bcgen Prkdctm1Bcgen Il2rgtm1Bcgen/Bcgen • 140739
| Product name | B-hSIRPA/hCD47,Prkdc/Il2rg KO mice |
|---|---|
| Catalog number | 140739 |
| Strain name | C57BL/6-Sirpatm1(SIRPA)Bcgen Cd47tm1(CD47)Bcgen Prkdctm1Bcgen Il2rgtm1Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 19261,16423,19090,16186 |
| Aliases | SIRPA: BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP; CD47: IAP, MER6, OA3;Prkdc: scid; Il2rg: CD132, [g]c, gamm, gamma(, gamma(c), gc, p64 |
Protein expression analysis
Species specific SIRPα and CD47 expression analysis in B-hSIRPA/hCD47,Prkdc/Il2rg KO mice by flow cytometry. Splenocytes were isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice (H/H) stimulated with anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-SIRPα and anti-CD47 antibodies. Human SIRPα and CD47 were exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 mice and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.
Analysis of leukocyte subpopulations in spleen
Analysis of leukocyte subpopulations in spleen by FACS. Splenocytes were isolated from C57BL/6, B-NDG mice and B-hSIRPA/hCD47,Prkdc/Il2rg KO mice (n=3, 11-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. Single live cells were gated for CD45 population and used for further analysis as indicated here. T, B and NK cells were undetectable in B-NDG mice and B-hSIRPA/hCD47,Prkdc/Il2rg KO mice.
