C57BL/6-Cd28tm1(CD28)Bcgen Tacstd2tm1(TACSTD2)Bcgen/Bcgen • 121252
| Product name | B-hCD28/hTROP2 mice |
|---|---|
| Catalog number | 121252 |
| Strain name | C57BL/6-Cd28tm1(CD28)Bcgen Tacstd2tm1(TACSTD2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | CD28, Tp44, CD28 molecule; TACSTD2, EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2, tumor associated calcium signal transducer 2, Trophoblast cell-surface antigen 2 |
Targeting strategyGene targeting strategy for B-hCD28/hTROP2 mice.
The exons 2-3 of mouse Cd28 gene that encode the extracellular domain were replaced by human CD28 exons 2-3 in B-hCD28/hTROP2 mice. The exon 1 of mouse Trop2 gene that encodes the full-length protein was replaced by human TROP2 exon 1 in B-hCD28/hTROP2 mice.
Protein expression analysis
Strain specific CD28 expression analysis in homozygous B-hCD28/hTROP2 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD28/hTROP2 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD28 antibody. Mouse CD28 was detectable in WT mice (+/+). Human CD28 was exclusively detectable in homozygous B-hCD28/hTROP2 mice (H/H) but not in WT mice (+/+).
Strain specific TROP2 expression analysis in homozygous B-hCD28/hTROP2 mice by western blot.
Skin tissue was collected from wild type (WT) mice (+/+) and homozygous B-hCD28/hTROP2 mice (H/H), and analyzed by western blot with anti-TROP2 antibody. Mouse TROP2 was detectable in WT mice (+/+). Human TROP2 was detectable in homozygous B-hCD28/hTROP2 mice (H/H) and B-hTROP2 MC38 cell line.
