C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)Bcgen Cd3gtm1(CD3G)Bcgen Tacstd2tm1(TACSTD2)Bcgen/Bcgen • 121633
| Product name | B-hCD3EDG/hTROP2 mice |
|---|---|
| Catalog number | 121633 |
| Strain name | C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)Bcgen Cd3gtm1(CD3G)Bcgen Tacstd2tm1(TACSTD2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | CD3E, IMD18, T3E, TCRE, CD3e molecule; CD3D, CD3-DELTA, IMD19, T3D, CD3d molecule; CD3G, CD3-GAMMA, IMD17, T3G, CD3g molecule); TACSTD2, EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2, tumor associated calcium signal transducer 2, Trophoblast cell-surface antigen 2 |
Targeting strategyGene targeting strategy for B-hCD3EDG/hTROP2 mice.
The exons 2-8 of mouse Cd3e gene were replaced by human CD3E exons 2-9 in B-hCD3EDG/hTROP2 mice. The exons 1-5 of mouse Cd3d and the exons 1-6 of Cd3g gene were replaced by human CD3D exons 1-5 and CD3G exons 1-7 in B-hCD3EDG/hTROP2 mice, respectively. The exon 1 of mouse Trop2 gene that encodes the full-length protein was replaced by human TROP2 exon 1 in B-hCD3EDG/hTROP2 mice.
mRNA expression analysis
Strain specific analysis of CD3D and CD3G gene expression in wild type (WT) mice and B-hCD3EDG/hTROP2 mice by RT-PCR.
Mouse Cd3d and Cd3g mRNA were detectable only in splenocytes of WT mice (+/+). Chimeric CD3D and CD3G mRNA were detectable only in homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific CD3E expression analysis in homozygous B-hCD3EDG/hTROP2 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H) but not in WT mice (+/+).
Strain specific TROP2 expression analysis in homozygous B-hCD3EDG/hTROP2 mice by western blot.
Skin tissue was collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H), and analyzed by western blot with anti-TROP2 antibody. Mouse TROP2 was detectable in WT mice (+/+). Human TROP2 was detectable in homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H) and B-hTROP2 MC38 cell line.
