C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Cd25tm1(CD25)Bcgen/Bcgen • 131078
| Product name | B-hPD-1/hPD-L1/hIL2RA mice |
|---|---|
| Catalog number | 131078 |
| Strain name | C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Cd25tm1(CD25)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 18566,60533,16184 |
| Aliases | PD-1 (Programmed death-1); CD274 (CD274 antigen); CTLA4 (Cytotoxic T-lymphocyte-associated protein 4,CD152) |
Protein expression analysis in T cells
Strain specific PD-1, PDL1 and IL2RA expression analysis in homozygous B-hPD-1/hPD-L1/hIL2RA mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hPD-L1/hIL2RA mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific PD-1, PD-L1 and IL2RA antibody. Mouse PD-1, PD-L1 and IL2RA were detectable in WT mice. Human PD-1, PD-L1 and IL2RA were exclusively detectable in homozygous B-hPD-1/hPD-L1/hIL2RA but not WT mice.
Protein expression analysis of IL2RA in Tregs
Strain specific IL2RA expression analysis in homozygous B-hPD-1/hPD-L1/hIL2RA mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hPD-L1/hIL2RA mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific IL2RA antibody. Mouse IL2RA were detectable in WT mice. Human IL2RA were exclusively detectable in homozygous B-hPD-1/hPD-L1/hIL2RA but not WT mice.
