B-hTEK mice

C57BL/6-Tektm1(TEK)Bcgen/Bcgen • 111106

B-hTDGF1 mice
B-hTEK/hTIE1 mice

B-hTEK mice

Product nameB-hTEK mice
Catalog number111106
Strain nameC57BL/6-Tektm1(TEK)Bcgen/Bcgen
Strain backgroundC57BL/6
AliasesTEK receptor tyrosine kinase, CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1

on this page

  • Targeting strategy
  • Phenotypic analysis

Posters

View All

    Publication

      Targeting strategy

      Gene targeting strategy for B-hTEK mice. The exons 2-14 of mouse Tek gene were replaced by human TEK exons 2-14 in B-hTEK mice.

      mRNA expression analysis
      Strain specific analysis of TEK gene expression in wild-type mice and B-hTEK mice by RT-PCR.Mouse Tek mRNA was detectable in the lung of wild-type mice (+/+). Human TEK mRNA was detectable only in homozygous B-hTEK but not in wild-type mice.
      Protein expression analysis
      Strain specific TEK expression analysis in homozygous B-hTEK mice by flow cytometry.Lung was collected from wild-type mice (+/+) and homozygous B-hTEK mice (H/H), and analyzed by flow cytometry with anti-TEK antibody. Mouse TEK was only detectable in wild-type mice. Human TEK was detectable in both homozygous B-hTEK mice and wild-type mice because of the cross-action antibody.